Cambridge · O Level
Biology past papers.
Every paper below is practisable online — timed simulations for multiple choice, mark-scheme self-marking for written papers.
91 papers
202512 papers
May/June 2025
PDFPaper 1 · Variant 1
15 multiple choice · 15 marks
Enzyme action · Blood vessels · Mammalian sense organs · Temperature control · Disease · Antibiotics · Nuclear division · Urinary system · Sexual reproduction in humans · Energy flow · Homeostasis · Effects of humans on ecosystems · Blood glucose control
Practice this paperMay/June 2025
PDFPaper 1 · Variant 2
17 multiple choice · 17 marks
Circulatory system · Photosynthesis · Effects of temperature and pH · Nuclear division · Disease · Mammalian sense organs · Inheritance · Effects of humans on ecosystems · Mammalian hormones · Features of organisms · Human digestive system · Aerobic respiration · Heart · Blood
Practice this paperMay/June 2025
PDFPaper 2 · Variant 1
32 structured · 78 marks
Disease · DNA · Energy flow · Sexual reproduction in plants · Immunity · Inheritance · Selection · Mineral nutrition · Photosynthesis · Human digestive system · Absorption and assimilation · Nutrient cycles · Biotechnology · Enzyme action · Effects of humans on ecosystems · Aerobic respiration · Biological molecules · Effects of temperature and pH
Practice questionsMay/June 2025
PDFPaper 2 · Variant 2
25 structured · 61 marks
Human gas exchange · Energy flow · Diet · Transpiration and translocation · Effects of temperature and pH · Immunity · Features of organisms · Nutrient cycles · DNA · Antibiotics · Effects of humans on ecosystems · Sexual reproduction in plants · Coordination and response in plants · Cell structure and function · Selection · Biotechnology
Practice questionsMay/June 2025
PDFPaper 4 · Variant 1
4 structured · 13 marks
Coordination and response in plants · Photosynthesis · Urinary system
Practice questionsMay/June 2025
PDFPaper 4 · Variant 2
7 structured · 19 marks
Enzymes · Coordination and response in plants
Practice questionsOctober/November 2025
PDFPaper 1 · Variant 1
12 multiple choice · 12 marks
Active transport · Respiration · Diet · Human gas exchange · Anaerobic respiration · Blood vessels · Sexual reproduction in plants · Nuclear division · Mammalian hormones · Selection · Conservation · Energy flow
Practice this paperOctober/November 2025
PDFPaper 1 · Variant 2
7 multiple choice · 7 marks
Diffusion and osmosis · Mineral nutrition · Diet · Mammalian nervous system · Biotechnology · Selection · Nutrient cycles
Practice this paperOctober/November 2025
PDFPaper 2 · Variant 1
5 structured · 12 marks
Circulatory system · Biotechnology · Effects of humans on ecosystems · Antibiotics
Practice questionsOctober/November 2025
PDFPaper 2 · Variant 2
7 structured · 20 marks
Effects of humans on ecosystems · Temperature control · Human digestive system · Effects of temperature and pH · Inheritance · Sexual reproduction in humans · Mammalian nervous system
Practice questionsOctober/November 2025
PDFPaper 3 · Variant 1
5 structured · 9 marks
Enzyme action · Biological molecules · Effects of temperature and pH · Leaf structure
Practice questionsOctober/November 2025
PDFPaper 3 · Variant 2
8 structured · 18 marks
Enzymes · Plant nutrition · Diffusion and osmosis
Practice questions
202412 papers
May/June 2024
PDFPaper 1 · Variant 1
15 multiple choice · 15 marks
Absorption and assimilation · Sexual reproduction in humans · Variation · Inheritance · Mammalian sense organs · Biological molecules · Anaerobic respiration · Enzyme action · Genetic modification · Ecosystems and biodiversity · Sexual reproduction in plants · Disease · Antibiotics
Practice this paperMay/June 2024
PDFPaper 1 · Variant 2
28 multiple choice · 28 marks
Sexual reproduction in humans · Excretion · Cell structure and function · Effects of temperature and pH · Variation · Active transport · Anaerobic respiration · Circulatory system · Coordination and response in plants · DNA · Disease · Uptake and transport of water and ions · Effects of humans on ecosystems · Mammalian hormones · Selection · Biotechnology · Photosynthesis · Human gas exchange · Sexual reproduction in plants · Mammalian sense organs · Diffusion and osmosis · Biological molecules · Immunity · Energy flow · Heart · Human digestive system
Practice this paperMay/June 2024
PDFPaper 2 · Variant 1
19 structured · 43 marks
Disease · Biotechnology · Diet · Nuclear division · Concept and use of a classification system · Mammalian nervous system · Cell structure and function · Genetic modification · Specialised cells, tissues and organs · Active transport · Human gas exchange · Diffusion and osmosis · Human digestive system
Practice questionsMay/June 2024
PDFPaper 2 · Variant 2
15 structured · 33 marks
DNA · Features of organisms · Urinary system · Mineral nutrition · Uptake and transport of water and ions · Selection · Asexual and sexual reproduction · Sexual reproduction in plants
Practice questionsMay/June 2024
PDFPaper 3 · Variant 1
3 structured · 7 marks
Ecosystems and biodiversity · Biological molecules
Practice questionsMay/June 2024
PDFPaper 3 · Variant 2
9 structured · 16 marks
Ecosystems and biodiversity · Sexual reproduction in plants · Biological molecules · Mineral nutrition
Practice questionsMay/June 2024
PDFPaper 4 · Variant 1
4 structured · 9 marks
Respiration · Classification · Transport in humans
Practice questionsMay/June 2024
PDFPaper 4 · Variant 2
6 structured · 15 marks
Ecosystems and biodiversity · Biological molecules · Mineral nutrition
Practice questionsOctober/November 2024
PDFPaper 1 · Variant 1
14 multiple choice · 14 marks
Active transport · Human digestive system · Effects of temperature and pH · Mineral nutrition · Immunity · Antibiotics · Mammalian nervous system · Asexual and sexual reproduction · Sexual reproduction in plants · Variation · Genetic modification · Selection · Ecosystems and biodiversity · Effects of humans on ecosystems
Practice this paperOctober/November 2024
PDFPaper 1 · Variant 2
9 multiple choice · 9 marks
Biological molecules · Transpiration and translocation · Uptake and transport of water and ions · Absorption and assimilation · Disease · Mammalian hormones · Nuclear division · Temperature control · DNA
Practice this paperOctober/November 2024
PDFPaper 2 · Variant 1
8 structured · 21 marks
Transpiration and translocation · Enzyme action · Excretion · Effects of humans on ecosystems · Biological molecules · Immunity · Variation
Practice questionsOctober/November 2024
PDFPaper 2 · Variant 2
8 structured · 25 marks
Immunity · Blood glucose control · Mammalian sense organs · Coordination and response in plants · Movement into and out of cells · Selection · Genetic modification
Practice questions