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Cambridge · O Level · Biology

May/June 2025 — Paper 2 · Variant 1.

32 structured questions · 78 marks

Download question paper (PDF)Mark scheme — for enrolled students

The mark scheme comes with the Biology course.

Topics covered

  • Disease
  • DNA
  • Energy flow
  • Sexual reproduction in plants
  • Immunity
  • Inheritance
  • Selection
  • Mineral nutrition
  • Photosynthesis
  • Human digestive system
  • Absorption and assimilation
  • Nutrient cycles
  • Biotechnology
  • Enzyme action
  • Effects of humans on ecosystems
  • Aerobic respiration
  • Biological molecules
  • Effects of temperature and pH

Questions in this paper

  • Q1cDNA3 marks
  • Q1aBiological molecules3 marks
  • Q2a(i)Energy flow1 marksincludes a figure
  • Q2b(ii)Selection4 marks
  • Q2a(ii)Energy flow2 marksincludes a figure
  • Q2a(iii)Energy flow2 marksincludes a figure
  • Q2b(i)Aerobic respiration2 marks
  • Q3a(i)Human digestive system2 marksincludes a figure
  • Q3a(ii)Human digestive system3 marksincludes a figure
  • Q3b(i)Absorption and assimilation3 marksincludes a figure
  • Q3b(ii)Effects of temperature and pH3 marks
  • Q4aDisease2 marks
  • Q4b(iii)Immunity4 marks
  • Q4b(iv)Immunity3 marks
  • Q4b(i)Disease3 marks
  • Q4b(ii)Disease2 marks
  • Q5b(ii)Energy flow2 marks
  • Q5c(i)Mineral nutrition2 marks
  • Q5b(i)Photosynthesis2 marks
  • Q5c(ii)Nutrient cycles2 marksincludes a figure
  • Q5aEffects of humans on ecosystems3 marks
  • Q6a(ii)Sexual reproduction in plants1 marks
  • Q6bSexual reproduction in plants3 marks
  • Q6cSexual reproduction in plants3 marksincludes a figure
  • Q6a(i)Sexual reproduction in plants2 marks
  • Q7a(i)Inheritance1 marks
  • Q7a(ii)Inheritance1 marks
  • Q7b(iii)Inheritance1 marksincludes a figure
  • Q7b(ii)Inheritance1 marksincludes a figure
  • Q7b(i)Inheritance2 marksincludes a figure
  • Q8aBiotechnology6 marksincludes a figure
  • Q8bEnzyme action4 marksincludes a figure

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